Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos
نویسندگان
چکیده
منابع مشابه
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro development of somatic cell nuclear transfer (SCNT) embryos. Green fluorescent protein (GFP) gene was used as a foreign gene and a non-transfected somatic cell was utilized as a control karyoplast. Mon...
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Currently, controlled ovarian hyperstimulation protocols commonly provide embryos in excess of those needed for fresh transfer. Therefore, techniques have been developed to store these surplus embryos in liquid nitrogen (referred to as cryopreservation) for an indefinite period of time without significant compromise of their quality. Based on data from the Centers for Disease Control and Preven...
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The cryopreservation of mammalian oocytes and embryos has application to preserve genetic diversity and for in vitro fertilization protocols in humans. To date, cryopreservation techniques have been used in a variety of species, including rabbits [1, 2], cattle [3, 4], horses [5, 6], cats [7, 8], mice [9, 10] and ferrets [11], among others. However, because of the biological uniqueness of porci...
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Changes in the number of lipid droplets during meiotic maturation, fert i l ization and early development were histochemically examined in cultured porcine oocytes and embryos. The oocytes and embryos possessed Sudanophilic lipids composed of small (<2.5 μm), medium (2.54.9 μm) and large (≥5.0 μm) droplets. In oocytes soon after collection, the numbers of Sudanophilic lipid droplets with small...
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Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryonic genome activation (EGA). RNA polymerase II (RNA Pol II) is responsible for the synthesis of mRNAs and most small nuclear RNAs, and consists of 12 subunits, the largest of which characteristically...
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ژورنال
عنوان ژورنال: Animals
سال: 2021
ISSN: 2076-2615
DOI: 10.3390/ani11020578